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| Registros recuperados: 14 | |
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| Melo,I.R.; Pimentel,M.F.; Lopes,C.E.; Calazans,G.M.T.. |
| Levan is a non-toxic, biologically active, extra cellular polysaccharide composed solely by fructose units. Optimization of levan production by Zymomonas mobilis strain ZAG-12 employing a 2(4-1) fractional factorial design was performed to analyze the influence of the temperature (20, 25 e 30ºC) agitation (50, 75 e 100 rpm), and the initial concentrations of both sucrose (150, 200 e 250 g.L-1) and yeast extract (2.0, 3.5 e 5.0g.L-1) on final levan concentration. Aerobic fermentation was performed batchwise in 500mL Pyrex flasks for 72 hours. Biomass, ethanol, levan and sucrose were determined at beginning and also at end of the fermentations. The experiments showed that the final levan concentration depended on initial sucrose concentration, temperature... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Factorial design; Levan; Zymomonas mobilis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100010 |
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| Pan, Nicole Caldas; Vignoli, Josiane Alessandra; Baldo, Cristiani; Pereira, Hanny Cristina Braga; Silva, Rui Sérgio dos Santos Ferreira; Celligoi, Maria Antonia Pedrine Colabone. |
| The production of hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 with varying rates of pH (6.0, 7.0, 8.0), temperature (34; 37; 40°C), agitation (100, 150, 200 rpm), glucose (10, 20, 30 g L-1) and yeast extract concentration (10, 20, 30 g L-1) was evaluated by statistical approaches. The best conditions for the production of hyaluronic acid was pH 8.0, 37°C and 100 rpm in a medium containing 30 g L-1 glucose and yeast extract, for a production of 0.787 g L-1. Temperature, pH and yeast extract were significant variables (p < 0.05). Yeast extract and pH had a positive effect on the production of the polymer. Lactate, formate and acetate synthesis were also analyzed. Current assay showed the feasibility of statistical tools to optimize the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioquímica de microrganismos physical factors; Nutritional factors; Glycosaminoglycans; Factorial design; Microbial production. Fermentação. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28176 |
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| Mandelli,Fernanda; Yamashita,Fábio; Pereira,José L.; Mercadante,Adriana Z.. |
| A fractional factorial design 2(5-1) was used to evaluate the effect of temperature, pH, and concentrations of yeast extract, tryptone and Nitsch's trace elements on the biomass, total carotenoids and protection against singlet oxygen by carotenoid extracts of the bacterium Thermus filiformis. In addition, the carotenoid composition was determined by high-performance liquid chromatography connected to a diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). The production of biomass ranged from 0.113 to 0.658 g/L, the total carotenoid from 137.6 to 1,517.4 mg/g and the protection against singlet oxygen from 4.3 to 85.1 %. Results of the fractional factorial design showed that temperature had a negative effect on biomass production and a positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thermophile bacterium; Carotenoids; HPLC-DAD-MS/MS; Antioxidant capacity; Factorial design. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100014 |
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| Pellosi,Diogo S.; Batistela,Vagner R.; Souza,Vagner R. de; Scarminio,Ieda S.; Caetano,Wilker; Hioka,Noboru. |
| The development of drugs for photodynamic therapy (PDT) is an important area of research due to their growing use in medical applications. Therefore, it is important to develop new bioassay methods for PDT photosensitizers that are inexpensive, easy to handle and highly sensitive to environmental conditions. Xanthene dyes (fluorescein, rose bengal B, erythrosine B and eosin Y) with LED light sources were investigated using Artemia salina as a bioindicator of photodynamic activity. In this study, three factors were investigated: (i) photosensitizers concentration, (ii) the LED irradiation time and (iii) the waiting time between the addition of the photosensitizers and the beginning of the irradiation. To analyze the photo-killing of A. salina, it was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artemia salina; Factorial design; Photodynamic therapy; Bioassay; Xanthenes. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401267 |
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| Prestes,Rosa Cristina; Carneiro,Eliana Beleski Borba; Demiate,Ivo Mottin. |
| The main problems observed in cooked hams are bad sliceability and excessive fluid loss after cooking. To reduce these problems the industry uses non-meat ingredients such as soy protein and carrageenan, but under Brazilian law, it is not allowed to add starch or modified starch in hams. Three ingredients were tested in the present research: modified starch (0 to 2.0%), gum guar (0 to 0.30%) and hydrolyzed collagen (0 to 2.0%), following a 2³ full factorial design with five repetitions in the central point. The guar gum produced low resistance to reheating, however in losses by cooling, the results were adequate. The hydrolyzed collagen tested did not give satisfactory results, showing low acceptance due to formation of gel in the ham and high losses. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Texture; Factorial design; Non-meat ingredients. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000700027 |
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| SILVA,MARCOS R.F. DA; QUEIROZ,MARIA ELIANA L.R. DE; NEVES,ANTÔNIO A.; SILVA,ANTÔNIO A. DA; OLIVEIRA,ANDRÉ F. DE; OLIVEIRA,RENAN L. DE; AZEVEDO,MARIANE M.. |
| Abstract Biochar is a carbonaceous material that has excellent potential as a fertilizer and soil conditioner. However, there is a lack of information concerning the effects of the amount and particle size of this pyrogenic material on the soil sorption capacity. In this work, evaluation was made of changes in clomazone (CMZ) sorption in a Red Latosol following soil conditioning using different percentages (0.25, 0.5, and 1% w/w) of sugarcane biochar in three particle sizes (<106, 106-508, and 508-610 µm). The conditioned soils presented apparent sorption coefficients (Kd) up to 1300 times higher than that of pure soil, besides changes in the behavior of CMZ sorption. The biochar particle size and percentage influenced sorption of the herbicide as well... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apparent sorption coefficient; Batch experiment; External surface area; Factorial design; Freundlich isotherm; Herbicide retention. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703745 |
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| Menegat, Francieli Bortoluzzi; Baldo, Cristiani; Celligoi, Maria Antonia Pedrine Colabone; Buzato, João Batista. |
| Asparaginase is an enzyme used in clinical treatments as a chemotherapeutic agent and in food technology to prevent acrylamide formation in fried and baked foods. Asparaginase is industrially produced by microorganisms, mainly gram-negative bacteria. Zymomonas mobilis is a Gram-negative bacterium that utilizes glucose, fructose and sucrose as carbon source and has been known for its efficiency in producing ethanol, sorbitol, levan, gluconic acid and has recently aroused interest for asparaginase production. Current assay optimizes the production of Z. mobilis asparaginase by continuous fermentation using response surface experimental design and methodology. The studied variables comprised sucrose, yeast extract and asparagine. Optimized condition obtained... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sucrose; Asparagine; Yeast extract; Enzyme; Microbial production; Factorial design. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31057 |
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| Kirsch,Larissa de Souza; Macedo,Ana Júlia Porto de; Teixeira,Maria Francisca Simas. |
| ABSTRACT Edible mushroom species are considered as an adequate source of food in a healthy diet due to high content of protein, fiber, vitamins, and a variety of minerals. The representatives of Pleurotus genus are characterized by distinct gastronomic, nutritional, and medicinal properties among the edible mushrooms commercialized worldwide. In the present study, the growth of mycelial biomass of Pleurotus albidus cultivated in submerged fermentation was evaluated. Saccharose, fructose, and maltose were the three main carbon sources for mycelial biomass formation with corresponding yields of 7.28 g L−1, 7.07 g L−1, and 6.99 g L−1. Inorganic nitrogen sources did not stimulate growth and the optimal yield was significantly higher with yeast extract (7.98 g... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pleurotus albidus; Submerged fermentation; Mycelial biomass; Factorial design. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300658 |
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| Mores,Rúbia; Kunz,Airton; Steffens,Juliana; Dallago,Rogério Marcos; Benazzi,Toni Luis; Amaral,André Cestonaro do. |
| ABSTRACT Anaerobic biodigestion is an appropriate alternative for the treatment of swine wastewater due to its biogas generation properties and the possibility of its application as a source of energy for heating or electricity. However, digestate can still contain high levels of turbidity, organic carbon and nutrients and must be correctly managed as a biofertilizer, or treated to avoid any impact on the environment. Considering this, electrocoagulation (EC) shows promise as a technology because of its ease of handling and high efficiency in effluent remediation. This study aimed to evaluate the performance of EC in a batch system in the treatment of swine wastewater digestate. The wastewater used in the treatment was sampled from a 10 m3 biodigestor... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electrode distance; Voltage; Factorial design; Batch system. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500439 |
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| Registros recuperados: 14 | |
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